| Goalkeeper | |||||
|---|---|---|---|---|---|
| # | Name | Country | Birthday | ||
| 30 |  | Andy Gruenebaum |  | USA | 30.12.1982 |
| 1 |  | Will Hesmer | | USA | 23.11.1981 |
| 18 |  | Kenny Schoeni | | USA | 04.01.1984 |
| Defence | |||||
| # | Name | Country | Birthday | ||
| 23 |  | Eric Brunner | | USA | 12.02.1986 |
 | Chris Clements | | USA | 02.03.1987 | |
 | Paul Gerstenberger | | USA | 26.01.1987 | |
| 6 |  | Andy Iro |  | England | 26.11.1984 |
| 14 |  | Chad Marshall | | USA | 22.08.1984 |
| 8 |  | Duncan Oughton |  | New Zealand | 14.06.1977 |
| 4 |  | Gino Padula |  | Argentina | 11.07.1976 |
| 18 |  | Robbie Rogers | | USA | 12.05.1987 |
| 24 |  | Jed Zayner | | USA | 13.12.1984 |
| Midfield | |||||
| # | Name | Country | Birthday | ||
| 15 |  | Kevin Burns | | USA | 22.03.1985 |
| 16 |  | Brian Carroll | | USA | 20.07.1981 |
| 17 |  | Emmanuel Ekpo |  | Nigeria | 20.12.1987 |
| 26 |  | Cory Elenio | | USA | 14.08.1986 |
| 12 |  | Eddie Gaven | | USA | 25.10.1986 |
| 3 |  | Alex Grendi | | USA | 28.07.1987 |
| 2 |  | Frankie Hejduk | | USA | 05.08.1974 |
| 22 |  | Adam Moffat |  | Scotland | 15.05.1986 |
| 31 |  | Stanley Nyazamba |  | Zimbabwe | 06.01.1983 |
| 5 |  | Danny O'Rourke | | USA | 31.05.1983 |
| Forward | |||||
| # | Name | Country | Birthday | ||
| 7 |  | Guillermo Barros Schelotto | | Argentina | 04.05.1973 |
| 9 |  | Jason Garey | | USA | 19.07.1984 |
| 32 |  | Steven Lenhart | | USA | 28.08.1986 |
| 10 |  | Alejandro Moreno |  | Venezuela | 08.07.1979 |
| Manager | |||||
| # | Name | Country | Birthday | ||
 | Robert Warzycha |  | Poland | 20.08.1963 | |
| Ass. Coach | |||||
| # | Name | Country | Birthday | ||
 | Mike Lapper | | USA | 28.08.1970 | |
| Goalkeeper Coach | |||||
| # | Name | Country | Birthday | ||
 | Vadim Kirillov |  | Russia | 09.09.1964 | |
Columbus Crew