All-time best penalty takers
| # | Name | Matches | 11m | |
|---|---|---|---|---|
| 1 |  | Pavel Verbíř | 410 | 24 |
| 2 |  | Jaroslav Melichár | 249 | 12 |
| 3 |  | Martin Fillo | 71 | 7 |
| 4 |  | Michal Doležal | 292 | 6 |
| 5 |  | Přemysl Bičovský | 113 | 5 |
 | Michal Bílek | 100 | 5 | |
 | Pavel Horváth | 73 | 5 | |
 | Ajdin Mahmutović | 164 | 5 | |
| 9 |  | Matěj Hybš | 33 | 4 |
 | Daniel Trubač | 244 | 4 | |
| 11 |  | Edin Džeko | 45 | 3 |
 | Jakub Hora | 118 | 3 | |
 | Jan Krob | 158 | 3 | |
 | Jakub Mareš | 174 | 3 | |
 | Štěpán Vachoušek | 276 | 3 | |
| 16 |  | Michal Bílek | 80 | 2 |
 | Daniel Fila | 45 | 2 | |
 | Petr Fousek | 142 | 2 | |
 | Antonín Rosa | 17 | 2 | |
 | Filip Žák | 48 | 2 | |
| 21 |  | Roman Čerepkai | 32 | 1 |
 | Filip Dort | 24 | 1 | |
 | Abdallah Gning | 80 | 1 | |
 | Svatopluk Habanec | 27 | 1 | |
 | Filip Havelka | 29 | 1 | |
 | David Kalivoda | 40 | 1 | |
 | Pavel Kloucek | 22 | 1 | |
 | František Knebort | 16 | 1 | |
 | Francis Litsingi | 68 | 1 | |
 | Pavel Moulis | 100 | 1 | |
 | Karel Rada | 122 | 1 | |
 | Jakub Řezníček | 45 | 1 | |
 | Antonín Rosa | 151 | 1 | |
 | Jiří Šourek | 57 | 1 | |
 | Jiří Tupec | 27 | 1 | |
 | Ivan Voborník | 23 | 1 | |
 | Patrik Žitný | 106 | 1 |