All-time best penalty takers
| # | Name | Matches | 11m | |
|---|---|---|---|---|
| 1 |  | Radek Drulák | 104 | 21 |
| 2 |  | Roman Hanuš | 152 | 17 |
| 3 |  | Michal Kovář | 333 | 11 |
 | Miroslav Příložný | 86 | 11 | |
| 5 |  | Jan Maroši | 212 | 8 |
| 6 |  | Karel Rada | 88 | 7 |
| 7 |  | Mojmír Chytil | 109 | 5 |
 | Jan Kliment | 48 | 5 | |
 | Melinho | 90 | 5 | |
| 10 |  | Jiří Balcárek | 147 | 4 |
 | Lukáš Juliš | 53 | 4 | |
 | Filip Zorvan | 100 | 4 | |
| 13 |  | Martin Hudec | 141 | 3 |
 | Dominik Janošek | 38 | 3 | |
 | David Kobylík | 127 | 3 | |
 | Miroslav Kouřil | 88 | 3 | |
 | Martin Nešpor | 70 | 3 | |
 | Pablo González | 85 | 3 | |
 | Jakub Plšek | 121 | 3 | |
 | Patrik Siegl | 85 | 3 | |
 | Jiří Vaďura | 235 | 3 | |
| 22 |  | Jiří Hajský | 25 | 2 |
 | Marek Heinz | 97 | 2 | |
 | David Houska | 179 | 2 | |
 | Tomáš Janotka | 173 | 2 | |
 | Ladislav Kučerňák | 87 | 2 | |
 | Vladislav Lauda | 69 | 2 | |
 | Miroslav Mlejnek | 87 | 2 | |
 | Michal Ordoš | 153 | 2 | |
| 30 |  | Lukáš Bajer | 101 | 1 |
 | Miloš Beznoska | 85 | 1 | |
 | Daniel Rossi | 139 | 1 | |
 | Pavel Hapal | 156 | 1 | |
 | Marek Kaščák | 101 | 1 | |
 | Milan Kerbr | 14 | 1 | |
 | Radoslav Látal | 151 | 1 | |
 | Tomáš Malec | 15 | 1 | |
 | Ladislav Onofrej | 109 | 1 | |
 | Tomáš Randa | 149 | 1 | |
 | Ľubomír Reiter | 93 | 1 | |
 | Václav Sejk | 26 | 1 | |
 | Ebrima Singhateh | 10 | 1 | |
 | Michal Šmarda | 58 | 1 | |
 | Daniel Vašulín | 23 | 1 | |
 | Stanislav Vlček | 202 | 1 |